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Knockout of the peroxiredoxin 5 homologue PFAOP does not affect the artemisinin susceptibility of Plasmodium falciparum

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Published in: Scientific reports 7(2017) Artikel-Nummer 4410, 23 Seiten; volume:7; year:2017
Authors and Corporations: Djuika, Carine F. (Author), Staudacher, Verena (Author), Sanchez, Cecilia P. (Author), Lanzer, Michael (Author), Deponte, Marcel (Author)
Other Authors: Staudacher, Verena [Author] • Sanchez, Cecilia P. [Author] • Lanzer, Michael [Author] • Deponte, Marcel 1977- [Author]
Type of Resource: E-Book Component Part
Language: English
30 June 2017
Series: Scientific reports, 7(2017) Artikel-Nummer 4410, 23 Seiten
Source: Verbunddaten SWB
Lizenzfreie Online-Ressourcen
ISSN: 2045-2322
Summary: Artemisinins are the current mainstay of malaria chemotherapy. Their exact mode of action is an ongoing matter of debate, and several factors have recently been reported to affect an early stage of artemisinin resistance of the most important human malaria parasite Plasmodium falciparum. Here, we identified a locus on chromosome 7 that affects the artemisinin susceptibility of P. falciparum in a quantitative trait locus analysis of a genetic cross between strains 7G8 and GB4. This locus includes the peroxiredoxin gene PFAOP. However, steady-state kinetic data with recombinant PfAOP do not support a direct interaction between this peroxidase and the endoperoxide artemisinin. Furthermore, neither the overexpression nor the deletion of the encoding gene affected the IC50 values for artemisinin or the oxidants diamide and tert-butyl hydroperoxide. Thus, PfAOP is dispensable for blood stage parasite survival, and the correlation between the artemisinin susceptibility and chromosome 7 is probably based on another gene within the identified locus.
Item Description: Gesehen am 22.08.2017
ISSN: 2045-2322
DOI: 10.1038/s41598-017-04277-5