A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep

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Bibliographic Details
Journal Title:	The Journal of Immunology
Authors and Corporations:	Pridans, Clare, Davis, Gemma M., Sauter, Kristin A., Lisowski, Zofia M., Corripio-Miyar, Yolanda, Raper, Anna, Lefevre, Lucas, Young, Rachel, McCulloch, Mary E., Lillico, Simon, Milne, Elspeth, Whitelaw, Bruce, Hume, David A.
In:	The Journal of Immunology, 197, 2016, 6, p. 2297-2305
Type of Resource:	E-Article
Language:	English
published:	The American Association of Immunologists
Subjects:	Immunology Immunology and Allergy

Description
Summary:	<jats:title>Abstract</jats:title> <jats:p>Expression of Csf1r in adults is restricted to cells of the macrophage lineage. Transgenic reporters based upon the Csf1r locus require inclusion of the highly conserved Fms-intronic regulatory element for expression. We have created Csf1r-EGFP transgenic sheep via lentiviral transgenesis of a construct containing elements of the mouse Fms-intronic regulatory element and Csf1r promoter. Committed bone marrow macrophage precursors and blood monocytes express EGFP in these animals. Sheep monocytes were divided into three populations, similar to classical, intermediate, and nonclassical monocytes in humans, based upon CD14 and CD16 expression. All expressed EGFP, with increased levels in the nonclassical subset. Because Csf1r expression coincides with the earliest commitment to the macrophage lineage, Csf1r-EGFP bone marrow provides a tool for studying the earliest events in myelopoiesis using the sheep as a model.</jats:p>
Physical Description:	2297-2305
ISSN:	1550-6606 0022-1767
DOI:	10.4049/jimmunol.1502336